Sample preparation

• Isolation of cell walls from different plants
• Sequential extraction of polysaccharides from cell walls:
  • Pectin fractions: WSP, CSP,ASP
  • Hemicelluloses fractions
  • Cellulose
  • Extraction of natural compounds with ASE 150 with solvents: ethyl alcohol, acetone, water
  • Sample lyophilisation
  • Dry sample grinding to the particle size of c.a. 6µm.
  • Microbiological medium and small laboratory equipment sterilization

Traditional chemical analyses

• Dry mass marking
• Acidity
• pH
• Extract contents
• Starch contents, iodine test, enzyme method, polymetric method
• Marking the level of pectin estrification with a spectrophotometric method
• Enzyme activity - a spectrophotometric method
  • Polygalacturonase PG
  • Alfa – L – arabinofuranosidase (Alfa – L –Af)
  • Beta – galactosidase (Beta – Gal)
  • Pectin methylesterase PME

Flow analysis

• Marking the contents of galacturonic acid in water solutions (1– 100 ppm C6H12O6)
• Marking the contents of calcium in water solutions (1-100 ppm Ca)
• Marking total nitrogen in samples after mineralization (0.1 – 10 ppm N)

FT Raman and FT IR

• Identification of organic and inorganic compounds
• Defining crystallization level of cellulose in cell walls

Granulometric analysis of powders

• 50µm, 100µm, 500µm, 1 mm, 2mm, 4mm.

Analytical services - microbiology

• Qualitative and quantitative marking of fruit and vegetable microflora,
• Gluconacetobacter xylinus bacteria breeding.

contact: Justyna Cybulska